CD147 / Emmprin / Basigin Antibody orb248197 from Biorbyt

Description

This antibody recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. CD147 stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis

Product Specs
ItemCD147 / Emmprin / Basigin Antibody
CompanyBiorbyt
Price Pricing Info Supplier Page
Catalog Numberorb248197
Quantity20 µg, 100 µg
ApplicationsFA, FACS, IF, IHC-P
Conjugate/TagUnconjugated
PurityProtein G purified CD147 antibody
ReactivityHuman
TargetCD147 / Emmprin / Basigin
IsotypeMouse IgG1, kappa
Clone8D6
HostMouse
ImmunogenHuman T cell line Molt-13 was used as the immunogen for this CD147 antibody
NCBI Full Gene Namebasigin (Ok blood group)
NCBI Gene Aliases5F7, CD147, EMMPRIN, EMPRIN, HAb18G, OK, TCSF
ClonalityMonoclonal
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageStore the CD147 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide)

Specifications/Features

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD147 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Application Dilutions: Inhibits homotypic aggregation, adhesion to matrix proteins, and migation through matrigel (order BSA/sodium azide-free format),Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1:100-1:200,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT

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